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2 edition of Cell cycle dynamics and the physiology of saxitoxin biosynthesis in Alexandrium fundyense (Dinophyceae) found in the catalog.

Cell cycle dynamics and the physiology of saxitoxin biosynthesis in Alexandrium fundyense (Dinophyceae)

Gaspar Taroncher Oldenburg

Cell cycle dynamics and the physiology of saxitoxin biosynthesis in Alexandrium fundyense (Dinophyceae)

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Published by Woods Hole Oceanographic Institution in Woods Hole, Mass .
Written in English

    Subjects:
  • Dinoflagellates.,
  • Saxitoxin.,
  • Cell cycle.

  • Edition Notes

    Statementby Gaspar Taroncher Oldenburg.
    SeriesMIT/WHOI -- 98-09., MIT/WHOI (Series) -- 98-09.
    ContributionsMassachusetts Institute of Technology., Woods Hole Oceanographic Institution.
    ID Numbers
    Open LibraryOL15533877M

    Culture, cell cycle synchronization, and flow-cytometric analysis. C. cohnii Biecheler cells were cultured in MLH medium at 28°C in the ynthetic Heterocapsa triquetra (CCMP) dinoflagellates were cultured in f/2 medium at 17°C under daily cycles of 14 h of light and 10 h of darkness.C. cohnii cells were synchronized at early G 1 by the cyst release filtration method as.   In Alexandrium catenella, toxin content per cell peaked during the S phase.(80) In Alexandrium fundyense, the biosynthesis of saxitoxins was restricted to 8â 10 hours in G1 and dropped to undetectable levels in other phases of the cell cycle.(81) In the benthic dinoflagellate Prococentrum lima, dinophysistoxin-4 (DTX4) synthesis is initiated.


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Cell cycle dynamics and the physiology of saxitoxin biosynthesis in Alexandrium fundyense (Dinophyceae) by Gaspar Taroncher Oldenburg Download PDF EPUB FB2

Request PDF | Cell cycle dynamics and the physiology of saxitoxin biosynthesis in Alexandrium fundyense (Dinophyceae) / | "Funding was provided by grants.

The aim of this thesis was to analyze novel aspects of toxigenesis during the cell cycle in Alexandrium and to apply molecular techniques to gain new insights on the genetics and regulation of STX biosynthesis.

Synchronized cultures of A. fundyense were studied to determine the dynamics of toxin production throughout the cell : Gaspar Taroncher-Oldenburg. Cell cycle dynamics and the physiology of saxitoxin.

Dinoflagellates of the genus Alexandrium produce the neurotoxin saxitoxin (STX), responsible for paralytic shellfish poisoning (PSP) and accumulates in marine invertebrates. The recent identification of STX biosynthesis genes allowed us to investigate the expression of sxtA4 at different growth stages in Alexandrium catenella Group IV.

We found no significant differences in expression Cited by: 9. We propose a model of the cell cycle of A. fundyense in which progression through the cell cycle can be arrested at two different transition points located in G 1 and toxin production is induced by light during G 1. The restriction off toxin production to a relatively short segment of the cell cycle provides a tool for comparing cells that are Cited by:   We studied a Alexandrium fundyense bloom using DinoSL-based technique.

The metatranscriptome reveals a metabolically active A. fundyense population. Genes for N-scavenging, CO 2-concentrating and saxitoxin production were upregulated in bloom. fundyense may experience N- CO 2-limitation and oxidative stress during the bloom.

Nickel superoxide dismutase of A. fundyense. G. Taroncher-Oldenburg, D.M. Kulis, D.M. AndersonCoupling of saxitoxin biosynthesis to the G1 phase of the cell cycle in the dinoflagellate Alexandrin fundyense: temperature and nutrient effects Natural Toxins, 7 (), pp. Zhuang Y, Zhang H, Hannick L, Lin S () Cell cycle, saxitoxin, and proton pump rhodopsin: insight from gene expression profiling for an Alexandrium fundyense culture and a.

Dinoflagellates are microscopic aquatic eukaryotes with huge genomes and an unusual cell regulation. For example, most genes are present in numerous copies and all copies seem to be obligatorily transcribed. The consequence of the gene copy number (CPN) for final protein synthesis is, however, not clear.

One such gene is sxtA, the starting gene of paralytic shellfish toxin (PST) synthesis. Cell cycle dynamics and the physiology of saxitoxin biosynthesis in Alexandrium fundyense (Dinophyceae) /.

"Funding was provided by grants from the National Science Foundation (OCE and OCE. cell of Alexandrium fundyense, collected at four different stages of toxin biosynthesis during the cell cycle, using an iTRAQ-based quantitative proteomic approach.

Dynamics and physiology. A series of studies with synchronized cultures of Alexandrium fundyense has further shown that STX is accumulated during a discrete time period localized in the G 1 phase of the cell cycle (Fig. (Fig.1) 1). The combination of these two factors, nuclear genes and discontinuous toxigenesis, gave rise to the hypothesis that STX biosynthesis could be regulated at the transcriptional level, with the genes.

Saxitoxin is an alkaloid neurotoxin originally isolated from the clam Saxidomus giganteus in This group of neurotoxins is produced by several species of freshwater cyanobacteria and marine dinoflagellates.

The saxitoxin biosynthesis pathway was described for the first time in the s and, since then, it was studied in more than seven cyanobacterial genera, comprising 26 genes that form. Studies show that toxin biosynthesis is regulated by genes in dinoflagellates, and at least the interconverting enzymes are encoded by nuclear genes [].In A.

fundyense, PST production occurs during a discrete time period localized in the early G1 phase of the cell cycle [], and three genes encoding S-adenosylhomocysteine hydrolase (SAHH), methionine aminopeptidase (MAP), and a histone-like.

Dynamics and physiology of saxitoxin production by the dinoflagellates Alexandrium spp. Mar. Biol. – /BF Evolutionary acquisition and loss of saxitoxin biosynthesis in dinoflagellates: the second “core” gene, Toxin variability during the cell cycle of the dinoflagellate Alexandrium fundyense.

Limnol. G. Taroncher-Oldenburg, D.M. Kulis, D.M. AndersonCoupling of saxitoxin biosynthesis to the G1 phase of the cell cycle in the dinoflagellate Alexandrium fundyense: temperature and nutrients effects Nat.

Toxins, 7 (), pp. Cell cycle dynamics and the physiology of saxitoxin biosynthesis in Alexandrium fundyense (Dinophyceae) / it is very important to understand the eco-physiology of each Alexandrium.

Yunyun Zhuang, Huan Zhang and Senjie Lin, Cyclin B gene and its cell cycle-dependent differential expression in the toxic dinoflagellate Alexandrium fundyense Atama Group I/Clade I, Harmful Algae, /, 26, (), ().

PST biosynthesis in Alexandrium fundyense is regulated by light and mainly occurs in the G1 phase [16,17,18]. However, PST biosynthesis in Alexandrium tamarense occurs in the early stage of the S phase. Similarly, toxin synthesis in Alexandrium catenella does not occur in the G1 phase but in the S phase or G2/M phase of the cell cycle [20, Taroncher-Oldenburg et al.

proved that toxin production of A. fundyense occurred within the G 1 phase of the cell cycle (Taroncher-Oldenburg et al., ). They suggested that a high toxin content in slow growing cells could be explained by increasing duration of the G 1 phase.

The effect of metabolic inhibitor, 5-fluoro-2′-deoxyuridine (FUdR) on toxin production and the cell cycle of marine dinoflagellate, Alexandrium tamarense, was ed to untreated cells, FUdR at 3 μM (p cell proliferation and toxin production in a dose-dependent manner for A.

tamarense cultured in modified T 1 medium. The effect of metabolic inhibitor, 5-fluoro-2′-deoxyuridine (FUdR) on toxin production and the cell cycle of marine dinoflagellate, Alexandrium tamarense, was investigated.

Anderson DM, Kulis DM, Sullivan JJ, Hall S, Lee C (b) Dynamics and physiology of saxitoxin production by the dinoflagellates Alexandrium spp. Mar Biol (Berl) – doi: /BF CrossRef Google Scholar.

Alexandrium is a neurotoxin-producing dinoflagellate genus resulting in paralytic shellfish poisonings around the world. However, little is known about the toxin biosynthesis mechanism in study compared protein profiles of A.

catenella collected at different toxin biosynthesis stages (non-toxin synthesis, initial toxin synthesis and toxin synthesizing) coupled with the cell.

Coupling of saxitoxin biosynthesis to the G1 phase of the cell cycle in the dinoflagellate Alexandrium fundyense: temperature and nutrient effects. Natural Toxins 7: Taroncher-Oldenburg, G.

and D.M. Anderson. Hwang DF: Isolation of bacteria from toxic dinoflagellate Alexandrium minutum and their effects on algae toxicity. I: Notes on Alexandrium population dynamics. Identification of a saxitoxin biosynthesis gene with a history of frequent horizontal gene transfers. Initial sequencing and analysis of the human genome.

Anderson DM, Kulis DM, Sullivan JJ, Hall S () Toxin composition variations in one isolate of the dinoflagellate Alexandrium fundyense.

Toxicon – Google Scholar Anderson DM, Glibert PM, Burkholder JM () Harmful algal blooms and eutrophication: nutrient sources, composition, and consequences. Download Tanrilar Ve Insanlar full book in PDF, EPUB, and Mobi Format, get it for read on your Kindle device, PC, phones or tablets.

Tanrilar Ve Insanlar full free pdf books. STX biosynthesis and the cell cycle in synchronously growing A. show relative distributions of cells in the different cell cycle stages (G 1 [ ], S [], and G 2 plus M []).Positive toxin production rates (bars; μ TOX) were limited to the first 8 to 10 h of the G 1 cell cycle phase.

No toxin production was detected for the remainder of the cell cycle. Dinoflagellates are a major component of marine phytoplankton and many species are recognized for their ability to produce harmful algal blooms (HABs).

Scrippsiella trochoidea is a non-toxic, marine dinoflagellate that can be found in both cold and tropic waters where it is known to produce “red tide” events. Little is known about the genomic makeup of S.

trochoidea and a transcriptome. In this review we summarize the current state of knowledge regarding taxonomy, bloom dynamics, toxicity, autoecology, and trophic interactions, of saxitoxin producing dinoflagellates in this region.

The dinoflagellates Gymnodinium catenatum, Pyrodinium bahamense and several species of Alexandrium are saxitoxin producers, and have been responsible of paralytic shellfish poisoning in different. Oldenburg et al. [18] in a toxic dinoflagellate A.

fundyense,it was found that toxin biosynthesis was coupled to the G 1 phase of the cell cycle. They also found that observed varia-tions in toxin content were a result of increasing periods of biosynthetic activity. Toxin content is generally high in the. Fig. STX biosynthesis and the cell cycle in synchronously growing A.

show relative distributions of cells in the different cell cycle stages (G 1 [ ], S [⧫], and G 2 plus M [ ]). Positive toxin production rates (bars; μ TOX) were limited to the first 8 to 10 h of the G 1 cell cycle phase.

No toxin production was detected for the remainder of the cell cycle. Anderson DM, Kulis DM, Sullivan JJ (a) Dynamics and physiology of saxitoxin production by the dinoflagellates Alexandrium spp.

Mar Biol – Toxin composition variations in one isolate of the dinoflagellate Alexandrium fundyense. cell cycle dynamics and the physiology of saxitoxin biosynthesis in alexandrium fundyense dinophyceae; a time for everything; values in education and education in values; kornevoj cuvassko russkij slovar sravnennyj s jazykami i narecijami raznych narodov tjurkskago finskago i drugich plemen tschuwaschisch russisches etymologisches worterbuch russ.

lecture notes on human physiology; mending mens suits; the new rules of attraction; beat the dealer; чтобы знали и помнили; project appraisal; مدخل لدراسة القانون و تطبيق الشريعة ال; cell cycle dynamics and the physiology of saxitoxin biosynthesis in alexandrium fundyense dinophyceae.

Furthermore, EST analysis of Alexandrium fundyense strain CCMPoriginally isolated from the Gulf of Maine, revealed libraries encoding cyanate lyase also known as cyanase (cynS), which functions in the degradation of cyanate, a toxic nitrogen-containing compound, to.

Dynamics and physiology of saxitoxin production by the dinoflagellates Alexandrium spp. Marine Biology, Anderson, et al.

Toxin composition variations in one isolate of the dinoflagellate Alexandrium Fundyense. Toxin variability during the cell cycle of the dinoflagellate Alexandrium fundyense.

Limnol Oceanogr42, – [Google Scholar] Taroncher-Oldenburg, G; Kulis, DM; Anderson, DM. Coupling of saxitoxin biosynthesis to the G1 phase of the cell cycle in the dinoflagellate Alexandrin fundyense: temperature and nutrient effects. Anderson, D. Bloom dynamics of toxic Alexandrium species in the northeastern U.S.

Limnol. Toxin composition variations in one isolate of the dinoflagellate Alexandrium fundyense. Toxicon and D. Anderson. Toxin variability during the cell cycle of the dinoflagellate Alexandrium fundyense. Limnol.

Oceanogr. Download Krisenwahrnehmungen In Deutschland Um full book in PDF, EPUB, and Mobi Format, get it for read on your Kindle device, PC, phones or tablets. Krisenwahrnehmungen In Deutschland Um full free pdf books.

In contrast to the biosynthesis of PSP toxins in synchronized A. fundyense cells, which occurs in G1 phase of the cell cycle following a light-dependent transition (Taroncher-Oldenburg et al., ; Taroncher-Oldenburg and Anderson, ), spirolide biosynthesis in A.

ostenfeldii is restricted primarily to the G2 phase (John et al., ).Assisted in a joint ESP/Imaging Flow CytoBot (IFCB) experiment analyzing saxitoxin concentrations during the cell cycle of the harmful algae bloom-causing dinoflagellate, Alexandrium fundyenseTitle: Graduate Research Fellow at .